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MessagePosté le: Dim 25 Sep - 03:43 (2016)    Sujet du message: Bonded Mande Matthews Epub Converter Répondre en citant




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d680c458d3
W....(2006)..Identification..and..characterization..of..L-arabonate..dehydratase,..L-2-keto-3-deoxyarabonate..dehydratase,..and..L-arabinolactonase..involved..in..an..alternative..pathway..of..L-arabinose..metabolism...aeruginosa.PAO1.genome.is.a.member.of.the.fumarylacetoacetate.hydrolase.(FAH).superfamily.25.However,.the.genome.neighborhoods.of.Labrenzia.aggregata.IAM.12614.and.several.other.organisms.that.encode.a.LyxD.lack.the.putative.L-Kdl.dehydratase.from.the.FAH.family;.instead,.they.include.a.gene.that.encodes.a.member.of.the.DHDPS.family.that.likely.is.the.L-Kdl.dehydratase.The.previously.characterized.2-keto-3-deoxy-d-arabinonate.dehydratase.(D-Kda).in.the.FAH.superfamily.utilizes.Mg2+.to.stabilize.the.enolate.anion.intermediate.26.The.mechanism.of.the.putative.L-Kdl.dehydratase.reaction.in.the.DHDPS.superfamily.is.expected.to.involve.the.formation.of.a.Schiff.base.between.the.substrate.and.an.active.site.Lys.residue.27.The.kinetic.constants.for.the.FAH.superfamily.L-Kdl.dehydratases.from.P..S.;...Fedorov...A....The..purified..sample..was..loaded..onto..a..HiLoad..S200..16/60..PR..gel..filtration..column..equilibrated..with..buffer..containing..20..mM..HEPES..at..pH..7.5,..150..mM..NaCl,..5%..glycerol,..5..mM..MgCl2,..and..5..mM..DTT...The.up-regulated.genes.encode.a.member.of.the.amidohydrolase.(AH).superfamily.(IPR006992),.a.member.of.the.4-hydroxythreonine-4-phosphate.dehydrogenase.family.(IPR005255;.PdxA-like.protein),.LyxD.(IPR001354),.a.member.of.the.fumarylacetoacetate.hydrolase.superfamily.(IPR005255;.the.putative.L-Kdl.dehydratase),.and.a.dehydrogenase.(IPR016161;.the.putative.αKGS.dehydrogenase;.αKGSDH).Figure.4QRT-PCR.results..A..Published..online..2014..May..6...[PubMed]4...

The.reaction.mixture.contained.10.mM.cis,cis-muconate.and.0.1.M.MLE.in.50.mM.Tris-Cl,.pH.7.5,.and.0.1.mM.MnCl2.Stereochemical.Studies1H.NMR.spectra.(500.MHz).were.recorded.in.a.D2O.containing.buffer.at.37.C..Exp....The..3′-megaprimer..was..constructed..using..the..T7term..primer..and..a..sense..primer..encoding..the..desired..mutation...Thus,.the.structures.of.the.active.site.cavities.are.not.conserved.between.the.two.families.Figure.4Sequence.alignment.of.the.syn-.and.anti-MLEs.from.P..Natl....Crystallography..&..NMR..system:..A..new..software..suite..for..macromolecular..structure..determination...A.plausible.pathway.for.the.catabolism.of.l-lyxonate.is.shown.in.Scheme.2..

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MessagePosté le: Dim 25 Sep - 03:43 (2016)    Sujet du message: Publicité

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